Concepts in Confocal Microscopy

Section Overview:

Laser scanning confocal microscopy represents one of the most significant advances in optical microscopy ever developed, primarily because the technique enables visualization deep within both living and fixed cells and tissues and affords the ability to collect sharply defined optical sections from which three-dimensional renderings can be created. The principles and techniques of confocal microscopy are becoming increasingly available to individual researchers as new single-laboratory microscopes are introduced. Development of modern confocal microscopes has been accelerated by new advances in computer and storage technology, laser systems, detectors, interference filters, and fluorophores for highly specific targets.

Review Articles

References and Resources

Confocal Microscopy Web Resources

Laser scanning confocal microscopy (LSCM) is a tool that has been extensively utilized for inspection of semiconductors, is now becoming a mainstream application in cell biology. The links provided in this section from the Olympus Microscopy Resource Center web site offer tutorials, instrumentation, application notes, technical support, glossaries, and reference materials on confocal microscopy and related techniques.

Contributing Authors

David W. Piston - Department of Molecular Physiology and Biophysics, Vanderbilt University, 702 Light Hall, Nashville, Tennessee, 37212.

Kenneth R. Spring - Scientific Consultant, Lusby, Maryland, 20657.

Matthew Parry-Hill, Thomas J. Fellers, and Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.