Fluorochrome Data Table

As a guide to fluorophores for confocal and widefield fluorescence microscopy, the table presented below lists many commonly-used fluorochromes, with their respective peak absorption and emission wavelengths and suggested laser illumination sources. While the authors assume responsibility for the accurate reporting of the data as published in various reliable sources, several caveats must be given. Fluorochrome dyes are environmentally sensitive and different results will be obtained with different solvents and applications. In reviewing the literature, one will frequently find somewhat different data supplied for the identical fluorochrome. There are also, in many instances, several sub-varieties of a fluorochrome.

The Handbook of Fluorescent Probes and Research Chemicals, published by Molecular Probes, Inc. in Eugene, Oregon is a rich source of information about fluorochrome dyes. Also, consult the publications of Omega Optical and Chroma Technology.

Although the data presented in this table have been checked for accuracy, it is strongly urged that the properties for a particular fluorophore be confirmed by first consulting the dye manufacturer to secure a full specification of the spectral profiles for absorption and emission prior to launching experiments. In addition, the suggested lasers are intended as a reference guide only, and in many cases, alternative illumination sources may produce superior results.