Fluorochromes and Suitable Cubes

• DAPI and WU

  4',6-diamidino-2-phenylindole, dihydrochloride (DAPI)-stained nuclei with a WU near-ultraviolet fluorescence cube.

• Hoechst 33258 and WU

  The DNA-specific bis-amidazole fluorochrome Hoechst 33258-stained nuclei with a WU near-ultraviolet fluorescence cube.

• Bodipy and WB

  An organelle-specific (golgi apparatus) fluorochrome, Bodipy, a thiol-reactive conjugation probe with the wide-pass green WB fluorescence cube.

• AMCA and WU

  The fluorochrome AMCA (7-amino-4-methylcoumarin-3-acetic acid), a bright blue conjugate and antibody stain with a WU near-ultraviolet fluorescence cube.

• Fast Blue and WU

  Featured here is nerve tracing reagent Fast Blue in a retrograde labeling experiment with the near-ultraviolet WU fluorescence cube.

• Lucifer Yellow and WBV

  Nerve cell tracing reagent Lucifer Yellow with the WBV fluorescence cube with a short-wavelength visible excitation filter and a long pass barrier filter.

• DiO and WB

  The membrane-specific fluorochrome 3,3'-dipentyloxacarbocyanine iodide (DiO) used as a tracer with a wide-pass WB fluorescence cube.

• FITC and WIB

  Featured here is an antibody labeling with fluorescein isothiocyanate (FITC) using the WIB green long-pass fluorescence cube.

• Diaminido Yellow and NV

  The fluorochrome Diaminido Yellow filtered with the short-wavelength visible NV fluorescence cube.

• Fluo-3 and WIB

  The calcium indicator Fluo-3 used with the long-pass WIB green fluorescence cube.

• Calcein AM and WIB

  The esterase substrate Calcein AM (acetoxymethyl) is used as a probe for cellular viablility when coupled with the WIB long-pass fluorescence cube.

• Propidium Iodide and WG

  The DNA-binding intercalator propidium iodide is used to stain cell nuclei with the WG long-pass red filter fluorescence cube.

• Pararosaniline-Feulgen and WG

  The nuclei/nucleic acid stain Pararosaniline-Feulgen is used in a nuclear preparation with the WG long-pass red filter fluorescence cube.

• DiI and WIG

  Displayed in this section, a photomicrograph illustrates the use of membrane-specific DiI with the WIG fluorescence cube.

• Ethidium Bromide and WIG

  The well-studied DNA-specific nuclear stain Ethidium Bromide is used with the WIG fluorescence cube.

• Cy 3 and WIG

  The protein modification reagent Cy 3 is used in a cytoskeletal experiment with the WIG fluorescence cube.

• TRITC and WIG

  Antibody-specific Tetramethyl Rhodamine Isothiocyanate (TRITC) is used in an antibody labeling preparation with the WIG fluorescence cube.

• Rhodamine 123 and WIB

  Rhodamine 123 is useful as a cell-permeant probe for mitochondria and also for live-cell function. Here it is used with the long-pass WIB fluorescence cube.

• Allophycocyanin and WIY

  The antibody-specific fluorochrome Allophycocyanin (APC) is used in an antibody labeling experiment with the long-pass WIY red fluorescence cube.

• Texas Red and WIY

  The antibody-specific fluorochrome Texas Red is used in an antibody labeling experiment with the long-pass WIY red fluorescence cube.