Fluorescence Cubes - Hoechst 33258 & WU

The photomicrograph below illustrates the bis-amidazole nuclear-specific fluorochrome, Hoechst 33258 in a nuclear stain using the WU fluorescence cube. The spectra below show the excitation and emission spectra of the dye (green spectra).

Objective: APO 100X
Photoeyepiece: PE 2.5X
Fluorescence Cube: WU
Exposure Mode: SFL 1