Fluorochrome Data Tables

The data tables below contain reference information on fluorescence cubes offered by Olympus America, Inc. to support their microscopes. The codes for the excitation columns in Tables 2 and 4 and the cube code column in Table 3 are listed in Table 1. Table 2 is a listing of examples of cubes provided for U-URA microscopes. The authors wish to caution that we believe this data to be accurate, but it should always be verified by authorized Olympus representatives.

Filter Color Codes

Code	Filter Type
U	Ultraviolet
V	Violet
BV	Blue-Violet
B	Blue
IB	Interference Blue Filter
G	Green
IG	Immuno Gold Staining Method
BF	Brightfield, Phase Contrast, and DIC only

Table 1

Table 2 lists the excitation and barrier filters by cube along with the dichroic mirror used in each cube. Use this chart as an aid to guide you in selection of some of the Olympus U-URA cubes for applications listed in our fluorescence and fluorochrome data tables.

Olympus Cubes Offered for U-URA

Excitation	Cube	Excitation Filter(nm)	Dichroic Mirror(nm)	Barrier Filter
U	U-MWU	330-385	400	420--
U	U-MNU	360-370	400	420--
U	U-MNUBP	360-370	400	420-460
V	U-MNV	400-410	455	455--
BV	U-MWBV	400-440	455	475--
BV	U-MNBV	420-440	455	475--
B	U-MWB	450-480	500	515--
IB	U-MWIB	460-490	505	515IF--
IB	U-MNIB	470-490	505	515IF--
IB	U-MNIBBP	470-490	505	515-550
IB	U-MWIBBP	460-490	505	515-550
G	U-MSWG	480-550	570	590--
G	U-MWG	510-550	570	590--
G	U-MNG	530-550	570	590--
IG	U-MNIG	520-550	565	580IF--
IY	U-MWIY	545-580	600	610IF--

Table 2

Table 3 lists dichroic mirror/filter combinations for the Olympus BH-2 and Vanox-3 fluorescent illuminators. The authors believe this information to be correct but it should be verified by Olympus representatives. Refer to Table 1 for the codes in the first column of this table.

CODE	Cube	Dichroic Mirror	Exciter Filter	Barrier Filter	Supplementary Exciter Filter	Additional Barrier Filter
U	-DMU	DM400	UG1	L420		L435
						Y455
V	-DMV	DM455	BP405	Y455		Y495
						Y495
						515
BV	-DMBV	DM455	BP440	AFC	EL420	Y495
				+Y475		515
B	-DMB	DM500	BP490	AFC	EL455	B460
				+O515		530
						570
						590
						G520
IB	-DMIB	DM505	BP495	0515IF	EY475	B460
						530
						570
						590
G	-DMG	DM570	BP545	590	EO515	R610
					EO530
IGS	-HMIGS	(Half-Mirror)	L420 +PO (Polarizer)	AN + DP
BF	-BF	(Optical axis adjuster)	(Light beam shutter)	(Image position adjuster)	-----	-----

Table 3

Table 4 is a cross-reference for Olympus U-URA cubes to Olympus BH-2, Vanox-3, and IMT-2 cubes. It is advisable to consult the manufacturer's wavelength/transmission curve diagrams for exciter filters, dichroic mirrors and barrier filters to determine how they match the fluorochrome being used. The cube names and nanometer numbers refer to the Olympus U-URA cubes; the figures in parentheses refer to filters still current for BH-2, Vanox 3, and IMT-2 fluorescent cubes.

Excitation	Cube	Excitation Filter	Dichroic Mirror	Barrier Filter
U	U-MU	BP330-385	DM400	BA420
		(UG-1)		(L420)
V	U-MNV	BP400-410	DM455	BA455
		(BP405)		(Y455)
BV	U-MWBV	BP400-440	DM455	Ba475
		(BP440)		(Y475&AFC)
BV	U-MNBV	BP420-440	DM455	BA475
		(BP440&EL420)		(Y475&AFC)
B	U-MWB	BP450-480	DM500	BA515
		(BP490&EY455)		(O515&AFC)
IB	U-MWIB	BP460-490	DM505	BA515IF
		(BP495)		(O515IF)
IB	U-MNIB	BP470-490	DM505	BA515IF
		(BP495&EY455)		(O515IF)
G	U-MSWG	BP480-550	DM570	BA590
		(BP545)		(O590)
G	U-MWG	BP510-550	DM570	BA590
		(BP545&EO515)		(O590)
G	U-MNG	BP530-550	DM570	BA590
		(BP545&EO530)		(O590)

Table 4

Use this chart as an aid to guide you in selection of some of the Olympus U-URA cubes if you wish to cross-reference to the extensive listing of fluorochromes in our other data sections.