A thick section of rat tongue was triple stained with the nucleic acid fluorochrome DAPI, fluorescein isothiocyanate (FITC), and Texas Red to yield the striking confocal image presented above. Nuclei of the cells fluoresce a bright blue, while a high-affinity receptor for brain-derived neutrotrophic factor (TrkB) is stained green in the image. Protein gene products, distributed throughout the tissue, are stained red with the popular rhodamine derivative. The image was contributed by Shigeru Takami from the Department of Anatomy in the School of Health Science at Kyorin University, in Japan. |