This horizontal thin section of rat brain clearly reveals the prominent longitudinal fissure. The specimen was fixed, permeabilized, blocked with 10-percent normal goat serum, and then treated with a cocktail of rabbit anti-GFAP (astroglia and neural stem cells) and chicken anti-NF-H (neurons) primary antibodies followed by goat anti-rabbit and anti-chicken secondary antibodies (IgG) conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively. Nuclei in the tissue section were subsequently targeted with DRAQ5 (pseudocolored cyan), a far-red fluorescent DNA probe. Images were recorded with a 20x objective using a zoom factor of 1.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.