A lateral ventricle can be seen in the coronal thin section of rat brain presented in the digital image above, which was immunofluorescently labeled for heavy chain neurofilament subunits (neurons) and glial fibrillary acidic protein (astroglia and neural stem cells) with chicken anti-NF-H antibodies and rabbit anti-GFAP primary antibodies. The primary targets were visualized with goat anti-chicken and anti-rabbit secondary antibodies conjugated to Alexa Fluor 488 (green fluorescence emission) and Alexa Fluor 568 (red emission), respectively. Nuclear DNA was counterstained with DRAQ5 (pseudocolored cyan). Images were recorded with a 20x objective using a zoom factor of 1.3 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.