In a double immunofluorescence experiment, a coronal section of rat hypothalamus tissue (shown above) was treated with a cocktail of mouse anti-vimentin (intermediate filaments) and chicken anti-NF-H (neurofilaments) primary monoclonal antibodies followed by goat anti-mouse and anti-chicken secondary antibodies (IgG) conjugated to Alexa Fluor 488 and Alexa Fluor 568, respectively. The specimen was counterstained for nuclei with DRAQ5, a far-red fluorescent DNA probe. Images were recorded with a 20x objective using a zoom factor of 1.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the Alexa Fluor 488 image channel was pseudocolored blue, the Alexa Fluor 568 channel was pseudocolored green, and the DRAQ5 channel was colored with red.