Neurofilaments, which are intermediate filaments that are found in neuronal cells, were immunofluorescently labeled in the coronal rat brain section featured in this confocal image with a primary antibody directed against one of their protein subunits (NF-H) followed by a secondary antibody conjugated to Alexa Fluor 568. In addition, the intermediate filament protein vimentin was targeted with an anti-vimentin primary antibody visualized with a secondary antibody conjugated to Alexa Fluor 488. DRAQ5 served as a nuclear counterstain. Images were recorded with a 40x oil immersion objective using a zoom factor of 1.6 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the Alexa Fluor 568 image channel was pseudocolored green, the Alexa Fluor 488 channel was pseudocolored blue, and DRAQ5 was pseudocolored red.