Glial fibrillary acidic protein (GFAP) is a type III intermediate filament protein that is a primary structural element of the star-shaped cells known as astrocytes and neural stem cells. The protein was targeted in a coronal section of rat brain cerebral cortex by immunofluorescently labeling the specimen with primary rabbit anti-GFAP antibodies followed by goat anti-rabbit Fab fragments conjugated to Alexa Fluor 488. The section was also stained for neurofilaments with chicken anti-NF-H antibodies visualized with goat anti-chicken secondary antibodies conjugated to Alexa Fluor 568. Cell nuclei were counterstained with DRAQ5 (pseudocolored blue). Images were recorded with a 20x objective using a zoom factor of 1.4 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.