Glial fibrillary acidic protein (GFAP) and heavy chain neurofilament proteins were targeted in a thin section of rat cerebellum (shown above) with rabbit anti-GFAP and chicken anti-NF-H monoclonal antibodies followed by goat anti-rabbit and anti-chicken secondary antibodies conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively. The nuclear counterstain DRAQ5 (pseudocolored cyan) was subsequently employed in order to label cell nuclei. Images were recorded with a 10x objective using a zoom factor of 1.2 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.