In a double immunofluorescence experiment, the rat cerebellum sagittal section presented above was fixed, permeabilized, blocked with 10-percent normal goat serum, and then treated with a cocktail of mouse anti-GFAP (glial fibrillary acidic protein) and rabbit anti-beta-III-tubulin (specific to neurons) primary antibodies followed by goat anti-mouse and anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively. Nuclei in the specimen were subsequently targeted with DRAQ5 (pseudocolored cyan). Images were recorded with a 10x objective using a zoom factor of 1.4 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.