A rat cerebellum thin section was immunofluorescently labeled for heavy chain neurofilament subunits with chicken anti-NF-H monoclonal antibodies followed by goat anti-chicken secondary antibodies conjugated to Alexa Fluor 488. In addition, glial fibrillary acidic protein was targeted in the tissue section with rabbit anti-GFAP antibodies visualized with goat anti-rabbit secondaries conjugated to Alexa Fluor 568. A far-red fluorescent probe, DRAQ5, was used to counterstain cell nuclei. Images were recorded with a 10x objective using a zoom factor of 1.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the DRAQ5 image channel was pseudocolored red, while the Alexa Fluor 488 and Alexa Fluor 568 image channels were pseudocolored blue and yellow, respectively.