An adherent monolayer A-10 cell culture was labeled for the cytoskeletal filamentous actin and intracellular mitochondrial networks with Alexa Fluor 488 conjugated to phalloidin and MitoTracker Red CMXRos, respectively. Nuclei present in the myoblasts were counterstained with the far-red fluorescent DNA-probe DRAQ 5. Images were recorded with a 60x oil immersion objective using a zoom factor of 1.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the Alexa Fluor 488 image channel was pseudocolored red, while the MitoTracker Red CMXRos channel was pseudocolored yellow and the DRAQ 5 channel was pseudocolored cyan.