The adherent culture of A-10 myoblasts featured above was immunofluorescently labeled with anti-vinculin mouse monoclonal primary antibodies followed by goat anti-mouse IgG secondary antibodies conjugated to Alexa Fluor 647 (pseudocolored blue). In addition, the specimen was stained for DNA with the ultraviolet-absorbing probe Hoechst 33342 (pseudocolored cyan), for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin, and for mitochondria with MitoTracker Red CMXRos. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.5 and sequential scanning with the 405-nanometer spectral line of a blue diode laser, the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.