Olympus Olympus IX2 Research Microscopes .
Laser Scanning Confocal Microscopy
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FluoView FV300/BX61


The Olympus FluoViewTM FV500 is a point-scanning, point-detection, confocal laser scanning microscope designed for biological research applications. Excellent resolution, efficiency of excitation, intuitive user interface and affordability are key characteristics of the Olympus FV500. A fully automated confocal system, the FV500 may be configured with ultraviolet through infrared lasers and permits simultaneous collection of up to 5 detection channels. The FV500 scanning unit can be coupled to either the IX2 inverted or the BX2 upright research microscope platforms.

The Olympus FV500 specification sheet includes information about laser light sources, the scanning unit, microscope combinations, external illumination sources, host computer configuration and requirements, the applications software, and power consumption statistics.

FV500 Microscope Specifications
Laser Light Sources

  • Visible Laser Sources (Mounted on Laser Combiner)
    • Multi Argon Laser - 40 milliwatts (458, 488, 514 nanometers)
    • Argon Laser - 10 milliwatts (488 nanometers)
    • Krypton Laser - 10 milliwatts (568 nanometers)
    • Helium-Neon (green) Laser - 1 milliwatt (543 nanometers)
    • Helium-Neon (red) Laser - 10 milliwatts (633 nanometers)
    • Helium-Cadmium Laser - 6 milliwatts (442 nanometers)

  • Ultraviolet Laser Sources (Connected via Fiber Optics)
    • Blue Diode Laser - 25 milliwatts (405 nanometers)
    • Argon Laser - 40 milliwatts (351 nanometers)

  • IR Laser - Connect IR diode laser (CW750 nanometers, 2 milliwatts) to the IR port via fiber system
  • Laser Combiner - Each laser light path is equipped with a continuously variable neutral density filter or acousto-optic tunable filter (AOTF). All laser lines are combined to pass along the same fiber optic cable.
Scanning Unit

  • Scanning Method - Galvanometer mirror scanners (both x and y axes)
  • Field Number - 18
  • Pinhole Aperture - Continuously adjustable pinhole for each individual detection channel
  • Image Scanning Speed
    • Standard Scanning Mode: 256 x 256 pixels (0.45 seconds) to 2048 x 2048 pixels (10.835 seconds) - Simultaneous scanning up to 5 channels in this mode
    • Bi-Directional High-Speed Scanning Mode: 512 x 512 pixels (0.25 seconds)

  • Selection of Filters - Automatic selection
  • Scanning Modes
    • 1-dimension - Point scanning over time
    • 2-dimension (space) - x-y, x-z and free line-z
    • 2-dimension (time) - x-t and free line-t
    • 3-dimension (space) - x-y-z
    • 3-dimension (time) - x-y-t and x-z-t
    • 4-dimension - x-y-z-t
    • 360║ rotation of the x-y plane is available for all modes

  • Image Depth Resolution - 12-bit (4096 gray levels) per channel
  • Zoom - 1X - 10X (0.5X-step)
  • Z-drive - Step motor/Minimum step 10 nanometers (BX61, BX61WI, and IX81 combination), 25 nanometers (other microscope combination)
Microscopes

  • Upright - BX51, BX61, BX61WI
  • Inverted (Special Laser Safe Frame) - IX81FVBF, IX81FVBFUV (back port), IX81FVSF, IX71FVSF (side port)

External Transmitted Light Unit

  • Transmitted Light Illumination - External halogen light source connected to microscope via fiber cable
  • Transmitted Light Detector - External detector unit with built-in photomultiplier. Connected to microscope via fiber cable
Fluorescence Illumination Unit

  • Connects external mercury light source to microscope via fiber cable.
  • Standard for FV500-BX51, FV500-BX61, FV500-BX61WI; Optional for FV500-IX81, FV500-IX71
PC with Systems Control Boards

  • IBM PC-AT compatible
  • Operating System - Windows« NT4.0, Windows 2000
  • Memory - 1GB RAM or larger; CPU: Pentium« 4 1 GHz or higher
  • Hard disk - 40 GB or larger, Special I/F board
  • Image acquisition - PCI bus
  • Monitor - Selectable from 21, 19, 17-inch, 1280 X 1024, Full color (16.77 million colors)
FluoViewTM Application Software

  • Image Acquisition

    • Scan Settings - image size, scanning speed, zoom, panning
    • Real-time image calculation - Kalman filtering, peak integration, PAPP (Programmable Acquisition Protocol Processor)

  • Hardware Control - Laser, scanning unit, microscope
  • Image Display

    • Each image display - Single-channel side-by-side, merge, cropping, tiling, series (z/t)
    • LUT - Individual color setting, pseudo-color
    • Overlay - Lines, text, scale bar, etc.

  • Image Processing

    • Individual filter - Average, Low-pass, High-pass, Sobel, Median, Prewitt, 2D Laplacian, edge enhancement, etc.
    • Calculations - Inter-image, mathematical and logical, DIC back ground leveling

  • Image Analysis - Overview of fluorescence intensity within an area, histogram, perimeter measurement for user-assigned area, time-lapse measurement, etc., TIEMPO (Time course software)
  • 3D Visualization - 3D animation, left/right stereo pairs, red/green stereoscopic images and cross section
  • Others - Graphic-based help, Ratiometric analysis software (optional), trigger IN/OUT function (optional)
Power Consumption

  • Microscope (115 volts, 6 amps or 230 volts, 3 amps), scanning unit + PSU (115 volts, 3.5 amps or 230 volts, 2 amps), computer and monitor (115 volts, 4.5 amps or 230 volts, 10 amps), Argon Laser (115 volts, 10 amps or 230 volts, 5 amps), Multi Argon Laser (115 volts, 10 amps or 230 volts, 5 amps), Krypton Laser (230 volts, 20 amps), Helium Laser each (115 volts, 0.4 amps or 230 volts, 0.2 amps), UV Laser (230 volts, 30 amps), IR Laser (115 volts, 1 amp or 230 volts, 0.5 amps), Helium-Cadmium Laser (115 volts, 5 amps or 230 volts, 3 amps)

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