Olympus Olympus BX2 Excitation Balancers .
Laser Scanning Confocal Microscopy
FluoView FV300/BX61

The Olympus FluoViewTM FV500 is a point-scanning, point-detection, confocal laser scanning microscope designed for biological research applications. Excellent resolution, efficiency of excitation, intuitive user interface and affordability are key characteristics of the Olympus FV500. A fully automated confocal system, the FV500 may be configured with ultraviolet through infrared lasers and permits simultaneous collection of up to 5 detection channels. The FV500 scanning unit can be coupled to either the IX2 inverted or the BX2 upright research microscope platforms.

The FV500 scanning unit combines maximum optical efficiency with a capacity for up to five photomultipliers with independent pinholes for each channel. Images may be scanned in any pixel array size up to 2048 x 2048, with each channel digitized to 4096 gray levels (12-bit resolution), permitting observation of fine image detail. Images may also be acquired in an automated sequential mode in order to reduce spectral crosstalk between channels for multi-color images.

Included in the compactly-designed scanning unit are a fiber optic coupler for multiple incoming laser light sources, a beam collimator, laser adjustment neutral density filter turret, dichromatic mirrors, polarizer, x-y galvanometer-driven scan mirrors, pinhole turret, photomultipliers, beamsplitters, fluorescence filters, and a collector lens.

Independent Pinholes for Each Channel

Because the depth of confocal images depends on the fluorescent wavelength and the pinhole diameter, the FV500 employs an independent pinhole for each channel, ensuring the best possible confocal resolution with each fluorophore.

3 Laser Ports - Up to 6 Lasers

The Olympus FV500 scanning unit is equipped with 3 fiber optic ports for ultraviolet, visible light (up to 4 lasers) and near-infrared lasers, and provides the variety of laser light sources necessary for imaging the latest generation of fluorescence indicators and fluorescent probes.

Simultaneous 5-Channel Image Acquisition

With up to 4 photomultiplier (PMT) detection channels for confocal fluorescence, plus a separate external photomultiplier for transmitted light detection, the FV500 is capable of recording images with up to 5 channels simultaneously.

Flexibility with Dichromatic Mirrors and Barrier Filters

Olympus' unique design of barrier filters and dichromatic mirrors for excitation and emission beamsplitting enables easy exchange to adapt for the specific requirements of new applications.

Shockless Method

The FV500 scanning unit and special microscope tube feature a shockless operating method. For even greater effectiveness, they can be combined with the unique fixed stage and nosepiece focusing BX61WI microscope.

Inverted Microscope Back Port System

The back port of the IX81 inverted microscope can be coupled to the Olympus FV500 scanning unit to combine compact design with versatile performance. Positioning the scanner at the rear of the motorized inverted microscope ensures plenty of working space on both sides of the microscope. Connection to the bottom and right side ports makes it possible to use up to three video devices in addition to the confocal scanning unit.

Compatible with a Wide Range of Microscopes

The FV500 can be used with Olympus upright microscopes, including the BX51, BX51WI, BX61, BX61WI, AX80, as well as inverted IX series microscopes through the side, bottom, or rear ports.