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The Olympus FluoViewTM FV1000 is a next-generation imaging system designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens. In addition, the FV1000 offers a revolutionary synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1000 an ideal choice for FRAP, FLIP and photoactivation.
The FluoView 1000 specification sheet includes information about laser light sources, the scanning unit, microscope combinations, external illumination sources, host computer configuration and requirements, the applications software, and power consumption statistics.
FV1000 Microscope Specifications
Laser Light Sources
- Visible Light Lasers (Mounted on Laser Combiner)
- Adjustment Method - Continuously variable with AOTF (0.1 to 100 percent in 0.1-percent increments)
- Translation Mode - Laser off during retrace period
- Region of Excitation - Capable of laser intensity adjustment and laser wavelength selection for each region
- Multi Argon-Ion Laser - 30 milliwatts (457, 488, 515 nanometers)
- Helium-Neon (green) Laser - 1 milliwatt (543 nanometers)
- Helium-Neon (red) Laser - 10 milliwatts (633 nanometers)
- Violet Laser (Optional)
- Adjustment Method - Current control to diode
- Translation Mode - Laser off during retrace period
- Region of Excitation - Capable of laser intensity adjustment for each region
- Diode (Blue) Laser - 6 milliwatts (405 nanometers); 0.7 milliwatts (440 nanometers)
- Near-Infrared Laser (Optional)
- Wavelength Range - 700-980 nanometers
- Permissible Power - Less than 5 watts
- AOTF Laser Combiner - Each laser light path is equipped with a continuously variable neutral density filter or acousto-optic tunable filter (AOTF). All laser lines are combined to pass along the same fiber optic cable.
Scanning Unit
- Basic Configuration
- Standard - 3 channels + 1 transmitted light channel, 3 photomultipliers and 3 laser ports, additional optional light path available
- Filters - Ion deposition filters; 6 filters can be attached to each filter turret for excitation, spectroscopy, and emission
- Selectable Fluorescence Detector - Filter/Spectral choices
- Spectral Type - Equipped with independent diffraction grating and slit for channel 1 and channel 2
- Selectable Wavelength Range - 1 to 100 nanometers
- Wavelength Resolution - 2 nanometers
- Wavelength Change Speed - 1 millisecond per 100 nanometers
- Scanning Method - Two galvanometer mirror scanners
- Scanning Modes
- Number of Pixels - 128 x 128 to 2048 x 2048 (64 x 64 to 4096 x 4096 provided by software)
- Scanning Speed (pixel time) - 2 to 8 milliseconds (2 to 5 milliseconds provided by software)
- Dimensions - Time, Z, and wavelength (provided by software) (unrestricted combination)
- Line Scanning - Straight line, free line, and point scanning
- Photomultiplier Operation Modes - Analog accumulation and photon counting mode
- Pinhole - One common pinhole for all channels, pinhole diameter 50 to 800 microns (50 to 300 micrometers with multispectral option), variable in 0.5 micrometer increments
- Field Number (N.A.) - 18
- Zoom - 1x to 50x, in 0.5x increments
- Z-drive - Uses motorized focus module in the microscope, minimum increment of 0.01 micrometers
Compatible Microscopes
- Inverted - IX81
- Upright - BX61, BX61WI
- Anti-Vibration Table - Air cushion anti-vibration table
Transmitted Light Detector Unit
- Built-in transmitted light detector and light source, motorized exchange, connected to the microscope frame via fiber cable
External Fluorescence Illumination Unit
- External fluorescence mercury arc-discharge light source, built-in shutter, motorized exchange between laser light path and fluorescence illumination, connected to the microscope frame via fiber cable
Control Unit
- PC-AT (Windows) compatible
- Operating System - Windows® XP Professional (English version)
- Memory - 1 Gbyte RAM or larger
- CPU - Pentium 2 GHz or higher
- Hard Disk - 80 Gbyte or larger, Special I/F board (built-in PC)
- Graphic Board - ATI Radeon 9200
- Memory Media - Built-in CD-R/RW
- Monitor - SXGA 1280 x 1024, twin 19-inch monitors
Optional Detector Units
- Twin Scanner Unit
- Two galvanometer mirrors, pupil projection lens, built-in laser shutter
- Laser Port - 1 port, incident light via fiber for visible and ultraviolet laser
- For incident light, additional separate laser is necessary
- Control and application software and power supply unit required
- Non-Confocal Detector - Built-in two channel detectors, spectral dichromatic mirror plus barrier filter method for wavelength selection (attached to the optional light path in scanning unit)
- 4 Channel Detector Unit - 4 channel detector for addition of laser sources
- Fiber Port for Fluorescence Output - Equipped with PC connector, compatible fiber core 100 to 125 microns
Principal Software Functions
- Software - Basic software, application software
- Image Format - Multi TIFF format, 8/16 bit gray scale/index color, 24/32/48 bit full color, JPEG/BMP/TIFF/AVI images
- Image Acquisition
- Region Designation - Point, line, free line, clip, clip zoom (For incident, additional separate laser is necessary)
- Real-time Image Calculation - Kalman filtering, peak detection calculation processing
- 2-Dimension - XY, XT and Xl
- 3-Dimension - XYZ, XYT, XYl, XZT, XlZ
- 4-Dimension - XYZT, XlZT, XYlT
- On-time control function, protocol processor
- Image Display
- Image Display - Single-channel side-by-side, merge, trimming, live tiling, scanning details information, series (Z/T), pass and continuous
- LUT - Individual color settings, pseudo-color
- Comment - Graphic and text input
- 3-D Visualization and Observation - 3-D animation, left/right stereo pairs, red/green stereoscopic images and cross section (3-D and 2-D sequential operation function with application software)
- Fluorescence Separation - Fluorescence separation through spectroscopy, normal and blind modes (through application software)
- Image Processing
- Individual filter - Average, Low-pass, High-pass, Sobel, Median, Prewitt (with application software), 2-D Laplacian, edge enhancement, etc.
- Calculations - Inter-image, mathematical and logical, DIC background leveling
- Image Analysis - Overview of fluorescence intensity, area and perimeter measurement, time-lapse measurement
- Statistical Processing - 2-D data histogram display, co-localization
- Additional Options - Time course software, review station software, 3-D and 4-D software/LSM viewer, XY stage control software for multi time-lapse experiment
Power Consumption
- Microscope (6 amperes), scanning unit (6.2 amperes), computer (4.5 amperes), multi argon-ion laser (100 volts, 10 amperes), helium-neon laser (0.5 amperes each)