Glial fibrillary acidic protein (GFAP) is a type III intermediate filament protein that is a primary structural element of the star-shaped glial cells known as astrocytes and neural stem cells. The protein was targeted in a coronal section of rat medulla oblongata tissue by immunofluorescently labeling the specimen with primary rabbit anti-GFAP antibodies followed by goat anti-rabbit Fab fragments conjugated to Alexa Fluor 568. The section was also stained for neurofilaments (specific to neurons) with chicken anti-NF-H antibodies visualized with goat anti-chicken secondary antibodies conjugated to Alexa Fluor 488. Nuclear DNA was counterstained with the red-absorbing dye DRAQ5 (pseudocolored cyan). Images were recorded with a 10x objective using a zoom factor of 1.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.