A coronal thin section of rat brain featuring the dentate gyrus (presented above) was immunofluorescently labeled for heavy chain neurofilament subunits (present in neurons) with chicken anti-NF-H antibodies followed by goat anti-chicken secondary antibodies conjugated to Alexa Fluor 488 (green fluorescence). In addition, glial fibrillary acidic protein (GFAP), which is strongly and specifically expressed in various astroglia and neural stem cells, was targeted in the specimen with rabbit anti-GFAP monoclonal antibodies visualized with goat anti-rabbit antibodies conjugated to Alexa Fluor 568 (red emission). Nuclear DNA was counterstained with the red-absorbing probe DRAQ5 (pseudocolored blue). Images were recorded with a 20x objective using a zoom factor of 2.2 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.