A horizontal thin section of rat brain tissue featuring a blood vessel (shown above) was immunofluorescently labeled for microtubule associated protein 2, which is expressed only in the dendrites and perikarya of neurons, with chicken anti-MAP2 polyclonal antibodies followed by goat anti-chicken secondary antibodies conjugated to Alexa Fluor 488 (yielding green fluorescence emission). Astroglia and intermediate filaments were also targeted in the specimen with rabbit anti-GFAP and mouse anti-vimentin antibodies visualized with goat anti-rabbit and anti-mouse secondaries conjugated to Alexa Fluor 568 (red fluorescence) and Alexa Fluor 647 (pseudocolored magenta), respectively. Cell nuclei were counterstained with Hoechst 33342. Images were recorded with a 20x objective using a zoom factor of 1.6 and sequential scanning with the 405-nanometer spectral line of a blue diode laser, the 488-nanometer line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.