In order to visualize the F-actin and mitochondrial networks in a culture of human osteosarcoma cells, the culture was treated with BODIPY FL conjugated to phallacidin and MitoTracker Red CMXRos. The nuclei in the cells were counterstained with TO-PRO-3, a carbocyanine monomer with long-wavelength red fluorescence. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.