After transiently transfecting an adherent culture of RK13 rabbit kidney cells (DsRed2-Endoplasmic Reticulum; 10-percent transfection efficiency), the culture (illustrated above) was washed, fixed, permeabilized, and blocked with bovine serum albumen. The cells were subsequently labeled with Alexa Fluor 488 conjugated to phalloidin and counterstained with Hoechst 33342 (pseudocolored cyan). Images were recorded with a 60x oil immersion objective using a zoom factor of 5.5 and sequential scanning with the 405-nanometer spectral line of a blue diode laser, the 488-nanometer spectral line of an argon-ion laser, and the 543-nanometer line from a green helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.