The mitochondrial and cytoskeletal filamentous actin networks were targeted in the culture of Pl 1 UT fibroblasts presented in the digital image above with BODIPY FL conjugated to phallacidin (a mushroom toxin) and MitoTracker Orange CMTMRos, respectively. Nuclei in the cells were counterstained with the far-red fluorescent DNA probe DRAQ5. Images were recorded with a 60x oil immersion objective using a zoom factor of 3.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the BODIPY FL image channel was pseudocolored blue, while the MitoTracker Orange CMTMRos image channel was pseudocolored red and the DRAQ5 channel was pseudocolored green.