The monolayer culture of Indian Muntjac fibroblast cells shown in this confocal image was treated with MitoTracker Red CMXRos in medium containing 15 percent Cosmic calf serum, fixed with the same medium containing 3.7 percent paraformaldehyde, permeabilized with 0.2 percent Triton X-100, and then counterstained with DRAQ5 (pseudocolored cyan), targeting DNA in the cell nuclei. Images were recorded on a FluoView FV1000 with a 60x oil immersion objective using a zoom factor of 5.5 and sequential scanning with the 543-nanometer line from a green helium-neon laser and the 633-nanometer line of a red helium-neon laser. During the processing stage, the image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.