A log phase culture of Indian Muntjac cells was treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 647 and Alexa Fluor 488, respectively, to target the nuclear histone proteins and peroxisomes. In addition, the F-actin network was labeled with Alexa Fluor 568 conjugated to phalloidin, a phallotoxin isolated from the death cap mushroom (Amanita phalloides). Images were recorded on a FluoView FV1000 with a 60x oil immersion objective using a zoom factor of 3.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the Alexa Fluor 488 image channel was pseudocolored green, the Alexa Fluor 568 image channel was pseudocolored red, and the Alexa Fluor 647 channel was pseudocolored blue.