An adherent culture of Indian Muntjac deer skin fibroblast cells was fixed, permeabilized, blocked, and then treated with mouse primary antibodies that target alpha-tubulin, a major component of the microtubule network. The primaries were labeled with goat anti-mouse secondary antibodies conjugated to Alexa Fluor 488 in a solution containing TO-PRO-3 to simultaneously stain DNA. Images were recorded on a FluoView FV1000 with a 60x oil immersion objective using a zoom factor of 2.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser and the 633-nanometer line of a red helium-neon laser. During the processing stage, the Alexa Fluor image channel was pseudocolored green and the TO-PRO-3 channel was pseudocolored red.