The Indian Muntjac deer skin fibroblast cells presented in the digital image above were resident in an adherent culture stained for F-actin with Alexa Fluor 546 conjugated to phalloidin, and for DNA with the red-absorbing dye TO-PRO-3 (pseudocolored blue). In addition, the culture was immunofluorescently labeled with Alexa Fluor 488 conjugated to antibodies that target alpha-tubulin, a major component of the microtubule network. Images were recorded on a FluoView FV1000 with a 60x oil immersion objective using a zoom factor of 2.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, Alexa Fluor image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.