The mitochondrial network in cultured Madin-Darby ovine kidney cells was immunofluorescently labeled with primary anti-oxphos complex V inhibitor protein monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. In addition, the F-actin network was targeted with Alexa Fluor 594 (pseudocolored lavender) conjugated to phalloidin, and the nucleus was counterstained with TO-PRO-3. Images were recorded with a 60x oil immersion objective using a zoom factor of 3.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.