The adherent monolayer culture of Madin-Darby ovine kidney cells illustrated above was immunofluorescently labeled with primary mouse anti-oxphos complex V inhibitor protein antibodies, followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. The culture was subsequently stained with Alexa Fluor 594 conjugated to phalloidin to reveal details of the filamentous actin network, and TO-PRO-3 (pseudocolored cyan) for DNA in the nucleus. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.