The rhesus monkey kidney cells presented in this confocal image were resident in an adherent culture stained for F-actin with BODIPY FL conjugated to phallacidin, and for mitochondria with MitoTracker Red CMXRos (pseudocolored yellow). In addition, the specimen was counterstained for DNA with the red-absorbing dye TO-PRO-3. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above. View a larger version of this digital image. |