The single tahr ovary epithelial cell presented in the digital image above was resident in a culture treated with MitoTracker Deep Red 633 (pseudocolored magenta) and Alexa Fluor 568 conjugated to phalloidin, fluorescently labeling the mitochondrial network and F-actin, respectively. The nucleic acid stain YO-PRO-1 was utilized to counterstain cell nuclei. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.