Confocal Microscopy Image Gallery

Tahr Ovary Epithelial Cells (HJ1.Ov Line)

HJ1.Ov Cells

The tahr ovary epithelial cell culture appearing in this confocal image was labeled with Alexa Fluor 568 conjugated to phalloidin, targeting the F-actin cytoskeletal network. The cells were also stained with MitoTracker Deep Red 633 and YO-PRO-1, which preferentially bind with intracellular mitochondria and DNA in the cell nucleus, respectively. Images were recorded with a 60x oil immersion objective using a zoom factor of 3.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the Alexa Fluor 568 image channel was pseudocolored blue, the MitoTracker Deep Red 633 image channel was pseudocolored red, and the YO-PRO-1 was pseudocolored green.

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