An adherent monolayer culture of human cervical adenocarcinoma cells was fixed, permeabilized, blocked, and then treated with mouse primary antibodies that target alpha-tubulin, a major component of the microtubule network. The primaries were labeled with goat anti-mouse secondary antibodies conjugated to Alexa Fluor 488 in a solution containing TO-PRO-3 to simultaneously stain DNA. Images were recorded with a 60x oil immersion objective using a zoom factor of 1.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.