The HeLa cell culture shown in this confocal image was fixed, permeabilized, washed, and blocked with 10-percent normal goat serum in phosphate-buffered saline prior to immunofluorescent labeling with rabbit primary antibodies to giantin, a protein resident in the Golgi complex of mammalian cells. The culture was subsequently stained with a mixture of secondary antibodies conjugated to Alexa Fluor 647 (pseudocolored blue). In addition, the filamentous actin network was probed with Alexa Fluor 488 conjugated to phalloidin, and DNA was labeled with the nucleic acid stain SYTOX Orange (pseudocolored red). Images were recorded with a 60x oil immersion objective using a zoom factor of 1.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.