A log phase culture of GMMe cells was treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Texas Red and Alexa Fluor 488, respectively. The procedure enabled the visualization of the nuclear histone proteins (red emission) and cytoplasmic peroxisomes (green emission) present in the cells. Images were recorded with a 60x oil immersion objective using a zoom factor of 6.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser and the 543-nanometer line from a green helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.