The Golgi apparatus was targeted in the culture of mink endometrium epithelial cells appearing in the digital image above by treating the cells with rabbit (anti-giantin) primary antibodies, followed by secondary antibodies conjugated to Alexa Fluor 568 (pseudocolored cyan). The culture was also stained for mitochondria with MitoTracker Deep Red 633, and for the actin cytoskeleton with Alexa Fluor 488 conjugated to phalloidin. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.