The Mongolian gerbil lung cells appearing in this confocal image were resident in a culture labeled with Alexa Fluor 568 (pseudocolored blue) conjugated to phalloidin, targeting the F-actin cytoskeletal network, and MitoTracker Deep Red 633, which preferentially binds with intracellular mitochondria. DNA in the nucleus was counterstained with YO-PRO-1. Images were recorded with a 60x oil immersion objective using a zoom factor of 3.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.