An adherent monolayer GeLu cell culture (presented above) was labeled for cytoskeletal filamentous actin and mitochondria with Alexa Fluor 488 conjugated to phalloidin and MitoTracker Red CMXRos, respectively. Nuclei present in the fibroblast cells were counterstained with the red-absorbing DNA probe DRAQ5. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles, with the exception of MitoTracker Red CMXRos, which was pseudocolored yellow.