MitoTracker Red CMXRos is a derivative of X-rosamine that selectively binds with mitochondria. By treating a culture of fox lung cells (shown above) with the MitoTracker probe, details of the mitochondrial network were visualized. The fibroblasts were also stained with DRAQ5, a far-red fluorescent DNA probe, in order to image cell nuclei. Images were recorded with a 60x oil immersion objective using a zoom factor of 5.0 and sequential scanning with the 543-nanometer line from a green helium-neon laser and the 633-nanometer line of a red helium-neon laser. During the processing stage, the MitoTracker Red CMXRos image channel was pseudocolored red and the DRAQ5 image channel was pseudocolored cyan.