Nuclei in a culture of FoLu cells were stained with Hoechst 33342 and immunofluorescence was utilized to visualize the numerous nuclear pore complexes located on each nucleus. The cells were fixed, permeabilized, blocked with 10-percent normal goat serum, and treated with mouse anti-NPCP (nuclear pore complex protein) primary antibodies followed by goat anti-mouse secondary antibodies (IgG) conjugated to Alexa Fluor 647 (pseudocolored yellow). The mitochondrial network was also targeted by treating the fibroblasts with MitoTracker Red CMXRos. Images were recorded with a 60x oil immersion objective using a zoom factor of 4.5 and sequential scanning with the 405-nanometer spectral line of a blue diode laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.