A monolayer culture of African green monkey kidney cells (presented above) was labeled with a mitochondrion-selective stain, MitoTracker Deep Red 633 (pseudocolored blue), and a high-affinity nucleic acid stain, SYTOX Orange. In addition, the filamentous actin network was targeted with BODIPY FL conjugated to phallacidin (a toxin derived from the death cap mushroom). Images were recorded with a 60x oil immersion objective using a zoom factor of 2.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.