The culture of COS-7 kidney cells presented in the digital image above was labeled for mitochondria with MitoTracker Orange CMTMRos and for the cytoskeletal filamentous actin network with BODIPY FL conjugated to phallacidin, a cyclic peptide derived from the toxic death cap mushroom (Amanita phalloides). In addition, the cells were probed for DNA in the cell nucleus with TO-PRO-3, a monomeric cyanine nucleic acid stain. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.