The filamentous actin and mitochondrial networks were targeted in an adherent culture of COS-7 kidney cells with BODIPY FL conjugated to phallacidin and MitoTracker Orange CMTMRos, respectively. DNA in the cells was counterstained with the red-absorbing dye TO-PRO-3 (pseudocolored cyan). Images were recorded with a 60x oil immersion objective using a zoom factor of 4.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.