The first stage of mitosis, prophase, can be observed in this confocal image. The red-absorbing dye DRAQ5 that was utilized to target the DNA in a culture of BPAE cells reveals that the nucleic acid has condensed into chromosomes in the cell presented above. The culture that the cell was resident in was also treated with MitoTracker Orange CMTMRos in order to visualize the mitochondrial network. Images were recorded with a 60x oil immersion objective using a zoom factor of 4.0 and sequential scanning with the 543-nanometer line from a green helium-neon laser and the 633-nanometer line of a red helium-neon laser. During the processing stage, the DRAQ5 image channel was pseudocolored red and the MitoTracker Orange CMTMRos channel was pseudocolored green.