Details of the mitochondrial and filamentous actin networks were revealed in the cells shown in this confocal image by treating the culture they were resident in with MitoTracker Orange CMTMRos (pseudocolored yellow) and BODIPY FL (pseudocolored blue) conjugated to phallacidin, a mushroom toxin. The culture was also treated with the red-absorbing nuclear counterstain DRAQ5. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.