The proximity between the mitochondrial and F-actin networks was visualized for this image by treating a culture of bovine pulmonary artery endothelial cells with the mitochondrion-selective stain MitoTracker Red CMXRos and BODIPY FL conjugated to a phallotoxin (phalloidin). The far-red fluorescent DNA probe DRAQ5 was utilized as a nuclear counterstain. Images were recorded with a 60x oil immersion objective using a zoom factor of 3.0 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the BODIPY FL image channel was pseudocolored with red, while the MitoTracker Red CMXRos channel was colored green and the DRAQ5 channel was colored cyan.