A single Swiss mouse embryo fibroblast cell that was part of an adherent culture stained with Alexa Fluor 488 conjugated to the lectin concanavalin A is presented in the digital image above. The probe Concanavalin A selectively binds to alpha-mannopyranosyl and alpha-glucopyranosyl residues in glycoproteins (primarily in the endoplasmic reticulum). The specimen was also counterstained with TO-PRO-3, targeting DNA in the nuclei. Images were recorded with a 60x oil immersion objective using a zoom factor of 3.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, as well as the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.