Specimen Preparation Using Synthetic Fluorophores and Immunofluorescence

Adherent Mink Uterus Endometrium Epithelial Cell

Adherent Rat Thoracic Aorta Smooth Muscle Cell

Presented above is a widefield fluorescence image revealing the vimentin intermediate filament and filamentous actin networks present in adherent mink uterus endometrium epithelial cells (GMMe line). The cells were immunofluorescently labeled with mouse anti-vimentin primary antibodies followed by goat anti-mouse secondary antibodies conjugated to Texas Red-X. In addition, the specimen was counterstained with phalloidin conjugated to Alexa Fluor 488 and DAPI, targeting the actin network and nuclei, respectively. Images were recorded in grayscale with a QImaging Retiga Fast-EXi camera system coupled to an Olympus BX-51 microscope equipped with bandpass emission fluorescence filter optical blocks provided by Omega Optical. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

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